![]() The description of obtained bacterial colonies was similar to the description of Staphylococcus aureus. After the second part of the experiment, we isolated bacteria colonies by colony streaking technique and could study them further if we needed. So, we could calculate the concentration of bacteria in the original culture. The obtained results showed a little deviation for a fifth diluted tube due to less pipetted volume from the tube, but the overall results confirmed that the dilution factor was preserved till the last dilution. CFU/ml we can observe after spreading and incubating them on a plate. The first part of the laboratory work showed that the more we dilute, the less number of bacteria colonies i.e. We obtained Staphylococcus aureus broth culture as a sample for our “preparation of plates from tubes 4D, 5D, and 6D” and “preparing a streak plate” experiments. Our major purpose of this experiment is to learn these techniques and observe them in a practice. Fundamental Lab Skills - Serial Dilution, Dilutions and Solution PrepĪbstract: The basic techniques in a cell and molecular biology are aseptic technique, dilution, and colony streaking, which are crucial for laboratory works. ![]()
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